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The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Fast and easy procedure with no tedious pipetting steps.No additional RNase H digestion step required.
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Sensitive detection of as few as 10 copies of template.High cDNA yields due to high-affinity enzyme.High cDNA yields due to high-affinity enzymeįor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Synthesis of double-stranded cDNA for cloning The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mix can be stored at 2–8☌. The combination of a hot start and a unique qPCR buffer system ensures highly specific and sensitive real-time quantification of gDNA and cDNA targets. The QuantiTect SYBR Green PCR Kit provides highly specific quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using SYBR Green I detection. Available with or without uracil-N-glycosylase (UNG).Reliable quantification of low-abundance transcripts.Real-time quantification of genomic DNA or cDNA targetsįor real-time PCR and two-step RT-PCR using SYBR Green High PCR specificity with integrated hot start For convenience, the master mix can be stored at 2–8☌. The QuantiTect SYBR Green RT-PCR Kit is also supplied with an optimized RT mix for efficient and sensitive reverse transcription over a wide range of RNA template amounts. The dNTP mix includes dUTP, allowing optional treatment with UNG. The combination of a hot start and a unique qRT-PCR buffer system ensures highly specific and sensitive real-time quantification of RNA targets. The QuantiTect SYBR Green RT-PCR Kit provides highly specific quantification of RNA targets by real-time one-step RT-PCR using SYBR Green I detection. No need to optimize reaction and cycling conditions.Accurate quantification over several logs of template.Reliable quantification of low-abundance transcriptsįor one-step qRT-PCR using SYBR Green I for gene expression analysis The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts. All components that are required for fast and efficient reverse transcription are provided with the QuantiTect Reverse Transcription Kit, including Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, and a unique RT Primer Mix. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. No need to design RNA-specific primers or probes.cDNA synthesis from 5' and 3' regions of transcripts.cDNA synthesis and gDNA removal in only 20 minutes.High cDNA yields even from low-abundance transcriptsįor fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC-rich) templates, is also supplied. Together with optimized salt concentrations, this additive stabilizes specifically bound primers and enables efficient extension of all primers in the reaction without the need for optimization. The QIAGEN Multiplex PCR Kit is available in a convenient ready-to-use master mix format. The QIAGEN Multiplex PCR Master Mix includes HotStarTaq DNA Polymerase and a unique PCR buffer containing the novel synthetic Factor MP. Highly suited for many types of multiplex PCR applications.High specificity and sensitivity with a built-in hot start.The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.DNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTPġ0 min at 97 DegreeC 60 min at 94 DegreeCįor highly specific and sensitive multiplex PCR without optimization requirements The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most.